Why need clean room in microbiology laboratory?

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Clean Room in Microbiology Testing Lab

A cleanroom is a core component in microbiology.

A laboratory, in which almost every microbiological activity is carried out, is vulnerable to exposure to unwanted particles, these particles carry viable particles as well. The penetration of these particles into the test can manipulate the results of a test or an experiment. Thus, it is important to ensure that the laboratory is clean, in other words, the required environment in which these contaminants are controlled so that they are in accordance with the specifications of the laboratory.

This is what a cleanroom is in microbiology.

A cleanroom is a laboratory or any location where the nature, size and number of permitted particles are checked so that they do not become contaminants that stand in the way of the test. Before conducting any microbiological testing, it is an important first step to control the lab environment. Detail written procedure must be implemented to enter and exit lab. This will ensure that the microbiological load is due to loading on sample not from the contaminated surrounding environment.  

For example, a cleanroom is obtained in microbiology:

The air entering a cleanroom area is first filtered and then recirculated through HEPA (High Efficiency Particle Attenuation) filters, which helps to control the particles. In the same way surfaces of laboratory must be disinfected so if any apparatus touch with surface remain clean. Operator hands, body and head covered properly so that it will not contribute to shed contaminants including lint from clothing.

The clean room is important for sterility testing of the product. 

Cleanrooms play vital role in controlling ISO-class of many microbiological testing equipment i.e. LFCs and BSCs. Otherwise filter change frequency will be high hence test will be fail. In microbiology sterility media is critical for testing. In ambient environment 50% media will be wasted due to contaminants growth. Above all the most critical impact of uncontrolled microbiology lab environment is false results. 

Fig: 1 Contamination in uncontrolled environments

Fig: 2 Show Media preparation in controlled environment 

Fig: 3 Test performed in Control environment






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